Molecular Identification of Cryptococcus neoformans Isolates from House Environments of HIV-Infected Patients in an Urban Area, Indonesia: A First Report

Cryptococcus neoformans isolates were previously obtained from pigeon droppings in Jakarta. This study aimed to determine another source of infection and describe the supporting niche fungus. The occurrence C. was evaluated 110 samples decaying wood leaves, tree hollow debris, dust, bird droppings. Yeasts collected 22 houses HIV-infected patients. identified based on culture characteristics, an assimilation test, URA5 restriction fragment length polymorphism polymerase chain reaction. spatial analysis conducted geographical information system dominant house environmental factors. Seven 120 (5.83%) as neoformans, corresponding four (18.2%) houses. All environments patients with cryptococcal meningitis. Spearman’s correlation McNemar’s test revealed a significant association between cryptococcosis their environment. clinical 100% identical molecular techniques, indicating that acquired soil, leaves distribution factors terms estimating disease prevalence. demonstrates environment is for cryptococcosis.